EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.78 | TyrAAT1 overproduced in Escherichia coli | Arabidopsis thaliana |
1.3.1.78 | TyrAAT2 overproduced in Escherichia coli | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.78 | AMP | - |
Arabidopsis thaliana | |
1.3.1.78 | cis-aconitate | - |
Arabidopsis thaliana | |
1.3.1.78 | L-tyrosine | - |
Arabidopsis thaliana | |
1.3.1.78 | NADPH | - |
Arabidopsis thaliana | |
1.3.1.78 | prephenate | - |
Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.78 | 0.0102 | - |
NADP+ | pH 7.5, 25°C, TyrAAT1 | Arabidopsis thaliana | |
1.3.1.78 | 0.0147 | - |
NADP+ | pH 7.5, 25°C, TyrAAT2 | Arabidopsis thaliana | |
1.3.1.78 | 0.0526 | - |
arogenate | pH 7.5, 25°C, TyrAAT1 | Arabidopsis thaliana | |
1.3.1.78 | 0.0842 | - |
L-arogenate | pH 7.5, 25°C, TyrAAT2 | Arabidopsis thaliana | |
1.3.1.78 | 17 | - |
prephenate | - |
Arabidopsis thaliana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.78 | 66000 | - |
1 * 66000, recombinant TyrAAT1, SDS-PAGE | Arabidopsis thaliana |
1.3.1.78 | 67000 | - |
recombinant TyrAAT1, gel filtration | Arabidopsis thaliana |
1.3.1.78 | 600000 | - |
above, recombinant TyrAAT2 | Arabidopsis thaliana |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.78 | Arabidopsis thaliana | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.78 | TyrAAT1 | Arabidopsis thaliana |
1.3.1.78 | TyrAAT2 | Arabidopsis thaliana |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.78 | 73 | - |
- |
Arabidopsis thaliana |
1.3.1.78 | 142 | - |
- |
Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.78 | L-arogenate + NADP+ | rapid equilibrium random mechanism in which two dead-end complexes, enzyme-NADPH-arogenate and enzyme-NADP+-tyrosine are formed | Arabidopsis thaliana | L-tyrosine + NADPH + CO2 | - |
? | |
1.3.1.78 | additional information | TyrAAT1 is four times more efficient in catalyzing arogenate dehydrogenase reaction than TyrAAT2. TyrAAT2 presents a weak prephenate dehydrogenase activity whereas Tyr-AAT1 does not | Arabidopsis thaliana | ? | - |
? | |
1.3.1.78 | prephenate + NADP+ | - |
Arabidopsis thaliana | 3-(4-hydroxyphenyl)-2-oxopropanoate + CO2 + NADPH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.78 | monomer | 1 * 66000, recombinant TyrAAT1, SDS-PAGE | Arabidopsis thaliana |
1.3.1.78 | oligomer | recombinant TyrAAT2 | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.78 | arogenate dehydrogenase isoform 1 | - |
Arabidopsis thaliana |
1.3.1.78 | arogenate dehydrogenase isoform 2 | - |
Arabidopsis thaliana |
1.3.1.78 | TyrAAT | - |
Arabidopsis thaliana |
1.3.1.78 | TyrAAT1 | - |
Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.78 | 3.4 | - |
prephenate | - |
Arabidopsis thaliana | |
1.3.1.78 | 37.3 | - |
L-arogenate | pH 7.5, 25°C, TyrAAT2 | Arabidopsis thaliana | |
1.3.1.78 | 84.2 | - |
arogenate | pH 7.5, 25°C, TyrAAT1 | Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.78 | NADP+ | - |
Arabidopsis thaliana | |
1.3.1.78 | NADPH | - |
Arabidopsis thaliana |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.78 | 0.0075 | - |
L-tyrosine | pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
1.3.1.78 | 0.0081 | - |
L-tyrosine | pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
1.3.1.78 | 0.0142 | - |
L-tyrosine | pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
1.3.1.78 | 0.0166 | - |
L-tyrosine | pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
1.3.1.78 | 0.0539 | - |
NADPH | pH 7.5, 25°C, with 0.02 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
1.3.1.78 | 0.0588 | - |
NADPH | pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
1.3.1.78 | 2.4 | - |
prephenate | pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
1.3.1.78 | 4.2 | - |
prephenate | pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
1.3.1.78 | 5.8 | - |
cis-aconitate | pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
1.3.1.78 | 17.8 | - |
AMP | pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
1.3.1.78 | 20.2 | - |
prephenate | pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
1.3.1.78 | 22.5 | - |
prephenate | pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
1.3.1.78 | 25.3 | - |
cis-aconitate | pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
1.3.1.78 | 25.9 | - |
AMP | pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
1.3.1.78 | 27.7 | - |
cis-aconitate | pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
1.3.1.78 | 42.9 | - |
cis-aconitate | pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
1.3.1.78 | 73.9 | - |
AMP | pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
1.3.1.78 | 115 | - |
AMP | pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 | Arabidopsis thaliana |